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[Forum] Dr. Michel Simonneau, Centre Psychiatrie & Neurosciences, INSERM U894, Paris & Ecole Normale Supérieure Paris-Saclay, France

“ Intraneuronal transport abnormalities induced by brain-disease-related genetic risk factors: fluorescent nanoparticle tracking ”

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June 23, 2017 11:00 - 12:00


BSI Central Building 1F Seminar Room


Brain diseases such as autism and Alzheimer's disease (each inflicting >1% of the world population) involve a large network of genes displaying subtle changes in their expression. Abnormalities in intraneuronal transport have been linked to genetic risk factors found in patients, suggesting the relevance of measuring this key biological process. However, current techniques are not sensitive enough to detect minor abnormalities. Here we report a sensitive method to measure the changes in intraneuronal transport induced by brain-disease-related genetic risk factors using fluorescent nanodiamonds (FNDs). We show that the high brightness, photostability and absence of cytotoxicity allow FNDs to be tracked inside the branches of dissociated neurons with a spatial resolution of 12 nm and a temporal resolution of 50 ms. As proof of principle, we applied the FND tracking assay on transgenic mouse lines that mimic the slight changes in protein concentration (∼30%) found in the brains of patients. In both cases, we show that the FND assay is sufficiently sensitive to detect these changes. KTiPO4 (KTP) and SiC are materials known for their non-linear optical properties. Illuminated crystals - at the frequency f - can emit a light at the double frequency 2f. This property is called Second Harmonic Generation (SHG) and it is widely used in electro-optical modulators and lasers. We are using this property to tackle intraneuronal transport in deep tissues such as brain slices.

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Yukiko Goda [Yukiko Goda, Synaptic Plasticity and Connectivity ]
Name: Yukiko Goda
Phone: 6221/6222