Marmoset Neural Architecture
Brain Mapping, MRI technology, Genetically-Modified Marmosets
Our lab was the first in the world to successfully generate transgenic marmosets with germline transmission. Our gene modification techniques can be used in a variety of non-human primates. Through our study of genetically modified marmosets (including disease models), we aim to understand the higher cognitive functions that are unique to primates. Ultimately, we hope to apply our work in treating human neurologic diseases.
Little is known about the axonal connectivity and functional distribution in the marmoset brain. Thus, we also aim to create a structural and functional marmoset brain atlas by combining 9.4T MRI, tracer injection and Ca2+ imaging techniques.
Okano H, Miyawaki A, and Kasai K.: "Brain/MINDS: Brain-Mapping Project in Japan.", Philos Trans R Soc Lond B Biol Sci. (2015)
Izpisua Belmonte JC, Callaway EM, Churchland P, Caddick SJ, Feng G, Homanics GE, Lee K-F, Leopold DA, Miller CT, Mitchell JF, Mitalipov F, Moutri AR, Movshon JA, Okano H, Reynolds JH, Ringach D, Sejnowski TJ, Silva AC, Strick PL, Wu J, and Zhang F. (in alphabetical order): "Brains, Genes and Primates.", Neuron (2015)
Okano H, and Mitra P: "Brain-mapping projects using the common marmoset.", Neurosci Res, In Press (2015)
Hikishima K, and Okano H.: "Detection of nigrostriatal pathway loss in the Parkinson's disease model using diffusion MRI Preliminary results in the MPTP-treated marmoset. ", Radiology, In Press (2015)
Kuwako K, Kawase S, Nishimoto Y, and Okano H.: "Cadherin-7 regulates mossy fiber connectivity in the cerebellum.", Cell Reports, 9(1), 311-323 (2014)
Bae B-I, Tietjen I, Atabay KD, Evrony GD, Johnson MB, Asare E, Murayama A, Im K, Crosier M, Lisgo SN, Lindsay S, Šestan N, Bernard S. Chang BS, Barkovich J, Grant E, Topçu M, Politsky J, Okano H, Piao X, and Walsh CA: "Evolutionarily Dynamic Alternative Splicing of GPR56 Regulates Regional Cerebral Cortical Patterning.", Science, 343(6172), 764-768 (2014)
Naka-Kaneda H, Nakamura S, Igarashi M, Aoi H, Kanki H, Tsutsumi S, Aburatani H, Shimazaki T, and Okano H: "The miR-17/106-p38 axis is a key regulator of the neurogenic-to-gliogenic competence transition in developing neural stem/progenitor cells", Proc.Natl.Acad.Sci.USA, 111(4), 1604-1609 (2014)
Bundo M, Toyoshima M, Okada Y, Akamatsu W, Ueda J, Nemoto-Miyauchi T, Sunaga F, Toritsuka M, Ikawa D, Kakita A, Kato M, Kasai K, Kishimoto T, Nawa H, Okano H, Yoshikawa T, Kato T, and and Iwamoto K: "Increased L1 Retrotransposition in the neuronal genome in Schizophrenia", Neuron, 81(2), 306-313 (2014)
Kuwako K., Kakumoto K, Imai T, Igarashi M, Hamakubo T, Sakakibara S, Tessier-Lavigne M, Okano HJ, and Okano H.: "Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through post-transcriptional regulation of Robo3/Rig-1 expression", Neuron, 67(3), 407-421 (2010)
Sasaki E, Suemizu H, Shimada A, Hanazawa K, Oiwa R, Kamioka M, Sotomaru Y, Hirakawa R, Eto T, Shiozawa S, Maeda T, Ito R, Kito C, Yagihashi C, Kawai K, Miyoshi H, Tanioka Y, Tamaoki N, Habu S, Okano H, and Nomura T.: "Generation of transgenic non-human primates with germ line transmission", Nature, 459(7246), 523-527 (*H. Okano is the corresponding author in this paper) (2009)