RIKEN BRAIN SCIENCE INSTITUTE (理研BSI)

論文一覧

宮脇  敦史

宮脇 (みやわき)  敦史( あつし)(みやわき あつし), M.D., Ph.D.

細胞機能探索技術開発チーム / シニア・チームリーダー

研究室詳細に戻る
  1. 1

    Miyawaki A, Shcherbakova DM, and Verkhusha VV: "Red fluorescent proteins: chromophore formation and cellular applications.", Curr Opin Struct Biol (2012)

  2. 2

    Miyawaki A: "Proteins on the move: insights gained from fluorescent protein technologies.", Nat Rev Mol Cell Biol, 12(10), 656-68 (2011)

  3. 3

    Mori R, Ikematsu K, Kitaguchi T, Kim SE, Okamoto M, Chiba T, Miyawaki A, Shimokawa I, and Tsuboi T: "Release of TNF-α from macrophages is mediated by small GTPase Rab37.", Eur J Immunol (2011)

  4. 4

    Itoh Y, Palmisano R, Anilkumar N, Nagase H, Miyawaki A, and Seiki M: "Dimerization of MT1-MMP during cellular invasion detected by flourescence resonance energy transfer.", Biochem J (2011)

  5. 5

    Katayama H, Kogure T, Mizushima N, Yoshimori T, and Miyawaki A: "A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery.", Chem Biol, 18(8), 1042-52 (2011)

  6. 6

    Hama H, Kurokawa H, Kawano H, Ando R, Shimogori T, Noda H, Fukami K, Sakaue-Sawano A, and Miyawaki A: "Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain.", Nat Neurosci (2011)

  7. 7

    Jovic A, Wade SM, Miyawaki A, Neubig RR, Linderman JJ, and Takayama S: "Hi-Fi transmission of periodic signals amid cell-to-cell variability.", Mol Biosyst, 7(7), 2238-44 (2011)

  8. 8

    Harada T, Matsuishi K, Oishi Y, Isobe K, Suda A, Kawan H, Mizuno H, Miyawaki A, Midorikawa K, and Kannari F: "Temporal control of local plasmon distribution on Au nanocrosses by ultra-broadband femtosecond laser pulses and its application for selective two-photon excitation of multiple fluorophores.", Opt Express, 19(14), 13618-27 (2011)

  9. 9

    Nakajima Y, Kuranaga E, Sugimura K, Miyawaki A, and Miura M: "Nonautonomous apoptosis is triggered by local cell cycle progression during epithelial replacement in Drosophila.", Mol Cell Biol, 31(12), 2499-512 (2011)

  10. 10

    Miyawaki A: "Development of probes for cellular functions using fluorescent proteins and fluorescence resonance energy transfer.", Annu Rev Biochem, 80, 357-73 (2011)

  11. 11

    Hanada Y, Sugioka K, Shihira-Ishikawa I, Kawano H, Miyawaki A, and Midorikawa K: "3D microfluidic chips with integrated functional microelements fabricated by a femtosecond laser for studying the gliding mechanism of cyanobacteria.", Lab Chip, 11(12), 2109-15 (2011)

  12. 12

    Takemoto K, Matsuda T, McDougall M, Klaubert DH, Hasegawa A, Los GV, Wood KV, Miyawaki A, and Nagai T: "Chromophore-assisted light inactivation of HaloTag fusion proteins labeled with eosin in living cells.", ACS Chem Biol, 6(5), 401-6 (2011)

  13. 13

    Kojima T, Karasawa S, Miyawaki A, Tsumuraya T, and Fujii I: "Novel screening system for protein-protein interactions by bimolecular fluorescence complementation in Saccharomyces cerevisiae.", J Biosci Bioeng, 111(4), 397-401 (2011)

  14. 14

    Kawase S, Imai T, Miyauchi-Hara C, Yaguchi K, Nishimoto Y, Fukami S, Matsuzaki Y, Miyawaki A, Itohara S, and Okano H: "Identification of a novel intronic enhancer responsible for the transcriptional regulation of musashi1 in neural stem/progenitor cells.", Mol Brain, 4, 14 (2011)

  15. 15

    Hamamura Y, Saito C, Awai C, Kurihara D, Miyawaki A, Nakagawa T, Kanaoka MM, Sasaki N, Nakano A, Berger F, and Higashiyama T: "Live-cell imaging reveals the dynamics of two sperm cells during double fertilization in Arabidopsis thaliana.", Curr Biol, 21(6), 497-502 (2011)

  16. 16

    Ohsawa S, Sugimura K, Takino K, Xu T, Miyawaki A, and Igaki T: "Elimination of oncogenic neighbors by JNK-mediated engulfment in Drosophila.", Dev Cell, 20(3), 315-28 (2011)

  17. 17

    Ogura Y, Sakaue-Sawano A, Nakagawa M, Satoh N, Miyawaki A, and Sasakura Y: "Coordination of mitosis and morphogenesis: role of a prolonged G2 phase during chordate neurulation.", Development, 138(3), 577-87 (2011)

  18. 18

    Sakaue-Sawano A, Kobayashi T, Ohtawa K, and Miyawaki A: "Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication.", BMC Cell Biol, 12, 2 (2011)

  19. 19

    Tian Q, Oberhofer M, Ruppenthal S, Scholz A, Buschmann V, Tsutsui H, Miyawaki A, Zeug A, Lipp P, and Kaestner L: "Optical action potential screening on adult ventricular myocytes as an alternative QT-screen.", Cell Physiol Biochem, 27(3-4), 281-90 (2011)

  20. 20

    Mizuno H, Mal TK, Wälchli M, Fukano T, Ikura M, and Miyawaki A: "Molecular basis of photochromism of a fluorescent protein revealed by direct 13C detection under laser illumination.", J Biomol NMR, 48(4), 237-46 (2010)

  21. 21

    Miyawaki A: "Imaging: Visualizing a neuronal handshake.", Nat Chem Biol, 6(12), 885-6 (2010)

  22. 22

    Li X, Chung LW, Mizuno H, Miyawaki A, and Morokuma K: "Competitive mechanistic pathways for green-to-red photoconversion in the fluorescent protein Kaede: a computational study.", J Phys Chem B, 114(49), 16666-75 (2010)

  23. 23

    Jovic A, Howell B, Cote M, Wade SM, Mehta K, Miyawaki A, Neubig RR, Linderman JJ, and Takayama S: "Phase-locked signals elucidate circuit architecture of an oscillatory pathway.", PLoS Comput Biol, 6(12), e1001040 (2010)

  24. 24

    Takahashi Y, Tsuji O, Kumagai G, Hara CM, Okano HJ, Miyawaki A, Toyama Y, Okano H, and Nakamura M: "Comparative study of methods for administering neural stem/progenitor cells to treat spinal cord injury in mice.", Cell Transplant (2010)

  25. 25

    Morikawa H, Kim M, Mimuro H, Punginelli C, Koyama T, Nagai S, Miyawaki A, Iwai K, and Sasakawa C: "The bacterial effector Cif interferes with SCF ubiquitin ligase function by inhibiting deneddylation of Cullin1.", Biochem Biophys Res Commun, 401(2), 268-74 (2010)

  26. 26

    Adam V, Mizuno H, Grichine A, Hotta J, Yamagata Y, Moeyaert B, Nienhaus GU, Miyawaki A, Bourgeois D, and Hofkens J: "Data storage based on photochromic and photoconvertible fluorescent proteins.", J Biotechnol, 149(4), 289-98 (2010)

  27. 27

    Horikawa K, Yamada Y, Matsuda T, Kobayashi K, Hashimoto M, Matsu-ura T, Miyawaki A, Michikawa T, Mikoshiba K, and Nagai T: "Spontaneous network activity visualized by ultrasensitive Ca(2+) indicators, yellow Cameleon-Nano.", Nat Methods, 7(9), 729-32 (2010)

  28. 28

    Isobe K, Suda A, Hashimoto H, Kannari F, Kawano H, Mizuno H, Miyawaki A, and Midorikawa K: "High-resolution fluorescence microscopy based on a cyclic sequential multiphoton process.", Biomed Opt Express, 1(3), 791-797 (2010)

  29. 29

    Aoki R, Kitaguchi T, Oya M, Yanagihara Y, Sato M, Miyawaki A, and Tsuboi T: "Duration of fusion pore opening and the amount of hormone released are regulated by myosin II during kiss-and-run exocytosis.", Biochem J, 429(3), 497-504 (2010)

  30. 30

    Isobe K, Hashimoto H, Suda A, Kannari F, Kawano H, Mizuno H, Miyawaki A, and Midorikawa K: "Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy.", Biomed Opt Express, 1(2), 687-693 (2010)

  31. 31

    Aiba Y, Kometani K, Hamadate M, Moriyama S, Sakaue-Sawano A, Tomura M, Luche H, Fehling HJ, Casellas R, Kanagawa O, Miyawaki A, and Kurosaki T: "Preferential localization of IgG memory B cells adjacent to contracted germinal centers.", Proc Natl Acad Sci U S A, 107(27), 12192-7 (2010)

  32. 32

    Tsutsui H, Higashijima S, Miyawaki A, and Okamura Y: "Visualizing voltage dynamics in zebrafish heart.", J Physiol, 588(Pt 12), 2017-21 (2010)

  33. 33

    Hashimoto H, Isobe K, Suda A, Kannari F, Kawano H, Mizuno H, Miyawaki A, and Midorikawa K: "Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy.", Appl Opt, 49(17), 3323-3329 (2010)

  34. 34

    Lütcke H, Murayama M, Hahn T, Margolis DJ, Astori S, Zum Alten Borgloh SM, Göbel W, Yang Y, Tang W, Kügler S, Sprengel R, Nagai T, Miyawaki A, Larkum ME, Helmchen F, and Hasan MT: "Optical recording of neuronal activity with a genetically-encoded calcium indicator in anesthetized and freely moving mice.", Front Neural Circuits, 4, 9 (2010)

  35. 35

    Hasegawa JY, Ise T, Fujimoto KJ, Kikuchi A, Fukumura E, Miyawaki A, and Shiro Y: "Excited states of fluorescent proteins, mKO and DsRed: chromophore-protein electrostatic interaction behind the color variations.", J Phys Chem B, 114(8), 2971-9 (2010)

  36. 36

    Hirano M, Hoshida T, Sakaue-Sawano A, and Miyawaki A: "A poly(dimethylsiloxane)-based device enabling time-lapse imaging with high spatial resolution.", Biochem Biophys Res Commun, 392(3), 307-10 (2010)

  37. 37

    Mizuno H, Dedecker P, Ando R, Fukano T, Hofkens J, and Miyawaki A: "Higher resolution in localization microscopy by slower switching of a photochromic protein.", Photochem Photobiol Sci, 9(2), 239-48 (2010)

  38. 38

    Kanki H, Shimabukuro MK, Miyawaki A, and Okano H: ""Color Timer" mice: visualization of neuronal differentiation with fluorescent proteins.", Mol Brain, 3, 5 (2010)

  39. 39

    Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, and Miyawaki A: "Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein.", Mol Biol Cell, 21(1), 87-94 (2010)

  40. 40

    Li X, Chung LW, Mizuno H, Miyawaki A, and Morokuma K: "A theoretical study on the nature of on- and off-states of reversibly photoswitching fluorescent protein Dronpa: absorption, emission, protonation, and Raman.", J Phys Chem B, 114(2), 1114-26 (2010)

  41. 41

    Kobiyama K, Takeshita F, Jounai N, Sakaue-Sawano A, Miyawaki A, Ishii KJ, Kawai T, Sasaki S, Hirano H, Ishii N, Okuda K, and Suzuki K: "Extrachromosomal histone H2B mediates innate antiviral immune responses induced by intracellular double-stranded DNA.", J Virol, 84(2), 822-32 (2010)

  42. 42

    Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima S, and Miyawaki A: "Illuminating cell-cycle progression in the developing zebrafish embryo.", Proc Natl Acad Sci U S A, 106(49), 20812-7 (2009)

  43. 43

    Tsutsui H, Shimizu H, Mizuno H, Nukina N, Furuta T, and Miyawaki A: "The E1 mechanism in photo-induced beta-elimination reactions for green-to-red conversion of fluorescent proteins.", Chem Biol, 16(11), 1140-7 (2009)

  44. 44

    Tomura M, Mori YS, Watanabe R, Tanaka M, Miyawaki A, and Kanagawa O: "Time-lapse observation of cellular function with fluorescent probe reveals novel CTL-target cell interactions.", Int Immunol, 21(10), 1145-50 (2009)

  45. 45

    Miyawaki A: "Great expectations.", Science, 326(5951), 339 (2009)

  46. 46

    Morikawa S, Mabuchi Y, Kubota Y, Nagai Y, Niibe K, Hiratsu E, Suzuki S, Miyauchi-Hara C, Nagoshi N, Sunabori T, Shimmura S, Miyawaki A, Nakagawa T, Suda T, Okano H, and Matsuzaki Y: "Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow.", J Exp Med, 206(11), 2483-96 (2009)

  47. 47

    Hashimoto J, Watanabe T, Seki T, Karasawa S, Izumikawa M, Seki T, Iemura S, Natsume T, Nomura N, Goshima N, Miyawaki A, Takagi M, and Shin-Ya K: "Novel in vitro protein fragment complementation assay applicable to high-throughput screening in a 1536-well format.", J Biomol Screen, 14(8), 970-9 (2009)

  48. 48

    Shimozono S, Tsutsui H, and Miyawaki A: "Diffusion of large molecules into assembling nuclei revealed using an optical highlighting technique.", Biophys J, 97(5), 1288-94 (2009)

  49. 49

    Isobe K, Suda A, Tanaka M, Kannari F, Kawano H, Mizuno H, Miyawaki A, and Midorikawa K: "Multifarious control of two-photon excitation of multiple fluorophores achieved by phase modulation of ultra-broadband laser pulses.", Opt Express, 17(16), 13737-46 (2009)

  50. 50

    Iwano M, Entani T, Shiba H, Kakita M, Nagai T, Mizuno H, Miyawaki A, Shoji T, Kubo K, Isogai A, and Takayama S: "Fine-tuning of the cytoplasmic Ca2+ concentration is essential for pollen tube growth.", Plant Physiol, 150(3), 1322-34 (2009)

  51. 51

    Isobe K, Suda A, Tanaka M, Hashimoto H, Kannari F, Kawano H, Mizuno H, Miyawaki A, and Midorikawa K: "Single-pulse coherent anti-Stokes Raman scattering microscopy employing an octave spanning pulse.", Opt Express, 17(14), 11259-66 (2009)

  52. 52

    Kikuchi A, Fukumura E, Karasawa S, Shiro Y, and Miyawaki A: "Crystal structure of a new cyan fluorescent protein and its hue-shifted variants.", Biochemistry, 48(23), 5276-83 (2009)

  53. 53

    Kobiyama K, Takeshita F, Ishii KJ, Koyama S, Aoshi T, Akira S, Sakaue-Sawano A, Miyawaki A, Yamanaka Y, Hirano H, Suzuki K, and Okuda K: "A signaling polypeptide derived from an innate immune adaptor molecule can be harnessed as a new class of vaccine adjuvant.", J Immunol, 182(3), 1593-601 (2009)

  54. 54

    Nishiumi F, Sone T, Kishine H, Thyagarajan B, Kogure T, Miyawaki A, Chesnut JD, and Imamoto F: "Simultaneous single cell stable expression of 2-4 cDNAs in HeLaS3 using psiC31 integrase system.", Cell Struct Funct, 34(1), 47-59 (2009)

  55. 55

    Miyawaki A: "Discovery of GFP.", Tanpakushitsu Kakusan Koso, 54(1), 78-84 (2009)

  56. 56

    Omran H, Kobayashi D, Olbrich H, Tsukahara T, Loges NT, Hagiwara H, Zhang Q, Leblond G, O'Toole E, Hara C, Mizuno H, Kawano H, Fliegauf M, Yagi T, Koshida S, Miyawaki A, Zentgraf H, Seithe H, Reinhardt R, Watanabe Y, Kamiya R, Mitchell DR, and Takeda H: "Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins.", Nature, 456(7222), 611-6 (2008)

  57. 57

    Miyawaki A: "Green fluorescent protein glows gold.", Cell, 135(6), 987-90 (2008)

  58. 58

    Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, and Miyawaki A: "Tracing the silhouette of individual cells in S/G2/M phases with fluorescence.", Chem Biol, 15(12), 1243-8 (2008)

  59. 59

    Kikuchi A, Fukumura E, Karasawa S, Mizuno H, Miyawaki A, and Shiro Y: "Structural characterization of a thiazoline-containing chromophore in an orange fluorescent protein, monomeric Kusabira Orange.", Biochemistry, 47(44), 11573-80 (2008)

  60. 60

    Asselberghs I, Flors C, Ferrighi L, Botek E, Champagne B, Mizuno H, Ando R, Miyawaki A, Hofkens J, Van der Auweraer M, and Clays K: "Second-harmonic generation in GFP-like proteins.", J Am Chem Soc, 130(46), 15713-9 (2008)

  61. 61

    Sanuki S, Hamanaka S, Kaneko S, Otsu M, Karasawa S, Miyawaki A, Nakauchi H, Nagasawa T, and Onodera M: "A new red fluorescent protein that allows efficient marking of murine hematopoietic stem cells.", J Gene Med, 10(9), 965-71 (2008)

  62. 62

    Matsunari H, Onodera M, Tada N, Mochizuki H, Karasawa S, Haruyama E, Nakayama N, Saito H, Ueno S, Kurome M, Miyawaki A, and Nagashima H: "Transgenic-cloned pigs systemically expressing red fluorescent protein, Kusabira-Orange.", Cloning Stem Cells, 10(3), 313-23 (2008)

  63. 63

    Tsutsui H, Karasawa S, Okamura Y, and Miyawaki A: "Improving membrane voltage measurements using FRET with new fluorescent proteins.", Nat Methods, 5(8), 683-5 (2008)

  64. 64

    Tomura M, Yoshida N, Tanaka J, Karasawa S, Miwa Y, Miyawaki A, and Kanagawa O: "Monitoring cellular movement in vivo with photoconvertible fluorescence protein "Kaede" transgenic mice.", Proc Natl Acad Sci U S A, 105(31), 10871-6 (2008)

  65. 65

    Fukano T, Shimozono S, and Miyawaki A: "Development of microscopic systems for high-speed dual-excitation ratiometric Ca2+ imaging.", Brain Cell Biol, 36(1-4), 43-52 (2008)

  66. 66

    Ueyama T, Kusakabe T, Karasawa S, Kawasaki T, Shimizu A, Son J, Leto TL, Miyawaki A, and Saito N: "Sequential binding of cytosolic Phox complex to phagosomes through regulated adaptor proteins: evaluation using the novel monomeric Kusabira-Green System and live imaging of phagocytosis.", J Immunol, 181(1), 629-40 (2008)

  67. 67

    Mizuno H, Mal TK, Wälchli M, Kikuchi A, Fukano T, Ando R, Jeyakanthan J, Taka J, Shiro Y, Ikura M, and Miyawaki A: "Light-dependent regulation of structural flexibility in a photochromic fluorescent protein.", Proc Natl Acad Sci U S A, 105(27), 9227-32 (2008)

  68. 68

    Kogure T, Kawano H, Abe Y, and Miyawaki A: "Fluorescence imaging using a fluorescent protein with a large Stokes shift.", Methods, 45(3), 223-6 (2008)

  69. 69

    Hanada Y, Sugioka K, Kawano H, Ishikawa IS, Miyawaki A, and Midorikawa K: "Nano-aquarium for dynamic observation of living cells fabricated by femtosecond laser direct writing of photostructurable glass.", Biomed Microdevices, 10(3), 403-10 (2008)

  70. 70

    Kawano H, Kogure T, Abe Y, Mizuno H, and Miyawaki A: "Two-photon dual-color imaging using fluorescent proteins.", Nat Methods, 5(5), 373-4 (2008)

  71. 71

    Matsuda T, Miyawaki A, and Nagai T: "Direct measurement of protein dynamics inside cells using a rationally designed photoconvertible protein.", Nat Methods, 5(4), 339-45 (2008)

  72. 72

    Sunabori T, Tokunaga A, Nagai T, Sawamoto K, Okabe M, Miyawaki A, Matsuzaki Y, Miyata T, and Okano H: "Cell-cycle-specific nestin expression coordinates with morphological changes in embryonic cortical neural progenitors.", J Cell Sci, 121(Pt 8), 1204-12 (2008)

  73. 73

    Hosoi H, Yamaguchi S, Mizuno H, Miyawaki A, and Tahara T: "Hidden electronic excited state of enhanced green fluorescent protein.", J Phys Chem B, 112(10), 2761-3 (2008)

  74. 74

    Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, and Miyawaki A: "Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.", Cell, 132(3), 487-98 (2008)

  75. 75

    Takeshita F, Kobiyama K, Miyawaki A, Jounai N, and Okuda K: "The non-canonical role of Atg family members as suppressors of innate antiviral immune signaling.", Autophagy, 4(1), 9月-67 (2008)

  76. 76

    Kaminuma O, Kitamura F, Kitamura N, Hiroi T, Miyoshi H, Miyawaki A, and Miyatake S: "Differential contribution of NFATc2 and NFATc1 to TNF-alpha gene expression in T cells.", J Immunol, 180(1), 319-26 (2008)

  77. 77

    Shimozono S, and Miyawaki A: "Engineering FRET constructs using CFP and YFP.", Methods Cell Biol, 85, 381-93 (2008)

  78. 78

    Katayama H, Yamamoto A, Mizushima N, Yoshimori T, and Miyawaki A: "GFP-like proteins stably accumulate in lysosomes.", Cell Struct Funct, 33(1), 1/12/11 (2008)

  79. 79

    Takahashi Y, Nishimura J, Suzuki A, Ishibashi K, Kinjo M, and Miyawaki A: "Cross-talk-free fluorescence cross-correlation spectroscopy by the switching method.", Cell Struct Funct, 33(1), 143-50 (2008)

  80. 80

    Habuchi S, Tsutsui H, Kochaniak AB, Miyawaki A, and van Oijen AM: "mKikGR, a monomeric photoswitchable fluorescent protein.", PLoS One, 3(12), e3944 (2008)

  81. 81

    Dedecker P, Hotta J, Flors C, Sliwa M, Uji-i H, Roeffaers MB, Ando R, Mizuno H, Miyawaki A, and Hofkens J: "Subdiffraction imaging through the selective donut-mode depletion of thermally stable photoswitchable fluorophores: numerical analysis and application to the fluorescent protein Dronpa.", J Am Chem Soc, 129(51), 16132-41 (2007)

  82. 82

    Flors C, Hotta J, Uji-i H, Dedecker P, Ando R, Mizuno H, Miyawaki A, and Hofkens J: "A stroboscopic approach for fast photoactivation-localization microscopy with Dronpa mutants.", J Am Chem Soc, 129(45), 13970-7 (2007)

  83. 83

    Miyawaki A, and Karasawa S: "Memorizing spatiotemporal patterns.", Nat Chem Biol, 3(10), 598-601 (2007)

  84. 84

    Miyawaki A: "Chemistry in studies of fluorescent proteins.", Tanpakushitsu Kakusan Koso, 52(13 Suppl), 1558-62 (2007)

  85. 85

    Miyawaki A, and Schnitzer MJ: "New technologies for neuroscience.", Curr Opin Neurobiol, 17(5), 565-6 (2007)

  86. 86

    Hayashi I, Mizuno H, Tong KI, Furuta T, Tanaka F, Yoshimura M, Miyawaki A, and Ikura M: "Crystallographic evidence for water-assisted photo-induced peptide cleavage in the stony coral fluorescent protein Kaede.", J Mol Biol, 372(4), 918-26 (2007)

  87. 87

    Miyawaki A: "Bringing bioluminescence into the picture.", Nat Methods, 4(8), 616-7 (2007)

  88. 88

    Takemoto K, Kuranaga E, Tonoki A, Nagai T, Miyawaki A, and Miura M: "Local initiation of caspase activation in Drosophila salivary gland programmed cell death in vivo.", Proc Natl Acad Sci U S A, 104(33), 13367-72 (2007)

  89. 89

    Jounai N, Takeshita F, Kobiyama K, Sawano A, Miyawaki A, Xin KQ, Ishii KJ, Kawai T, Akira S, Suzuki K, and Okuda K: "The Atg5 Atg12 conjugate associates with innate antiviral immune responses.", Proc Natl Acad Sci U S A, 104(35), 14050-5 (2007)

  90. 90

    Ando R, Flors C, Mizuno H, Hofkens J, and Miyawaki A: "Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants.", Biophys J, 92(12), L97-9 (2007)

  91. 91

    Tada J, Kono T, Suda A, Mizuno H, Miyawaki A, Midorikawa K, and Kannari F: "Adaptively controlled supercontinuum pulse from a microstructure fiber for two-photon excited fluorescence microscopy.", Appl Opt, 46(15), 3023-30 (2007)

  92. 92

    Fron E, Flors C, Schweitzer G, Habuchi S, Mizuno H, Ando R, Schryver FC, Miyawaki A, and Hofkens J: "Ultrafast excited-state dynamics of the photoswitchable protein Dronpa.", J Am Chem Soc, 129(16), 4870-1 (2007)

  93. 93

    Yamada M, Tanemura K, Okada S, Iwanami A, Nakamura M, Mizuno H, Ozawa M, Ohyama-Goto R, Kitamura N, Kawano M, Tan-Takeuchi K, Ohtsuka C, Miyawaki A, Takashima A, Ogawa M, Toyama Y, Okano H, and Kondo T: "Electrical stimulation modulates fate determination of differentiating embryonic stem cells.", Stem Cells, 25(3), 562-70 (2007)

  94. 94

    Truong K, Sawano A, Miyawaki A, and Ikura M: "Calcium indicators based on calmodulin-fluorescent protein fusions.", Methods Mol Biol, 352, 71-82 (2007)

  95. 95

    Tojima T, Akiyama H, Itofusa R, Li Y, Katayama H, Miyawaki A, and Kamiguchi H: "Attractive axon guidance involves asymmetric membrane transport and exocytosis in the growth cone.", Nat Neurosci, 10(1), 58-66 (2007)

  96. 96

    Fukano T, Sawano A, Ohba Y, Matsuda M, and Miyawaki A: "Differential Ras activation between caveolae/raft and non-raft microdomains.", Cell Struct Funct, 32(1), 9/15/11 (2007)

  97. 97

    Tsuruwaka Y, Konishi T, Miyawaki A, and Takagi M: "Real-time monitoring of dynamic intracellular Ca(2+) movement during early embryogenesis through expression of yellow cameleon.", Zebrafish, 4(4), 253-60 (2007)

  98. 98

    Chan MC, Karasawa S, Mizuno H, Bosanac I, Ho D, Privé GG, Miyawaki A, and Ikura M: "Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus.", J Biol Chem, 281(49), 37813-9 (2006)

  99. 99

    Hara C, Tateyama K, Akamatsu N, Imabayashi H, Karaki K, Nomura N, Okano H, and Miyawaki A: "A practical device for pinpoint delivery of molecules into multiple neurons in culture.", Brain Cell Biol, 35(4-6), 229-37 (2006)

  100. 100

    Hosoi H, Mizuno H, Miyawaki A, and Tahara T: "Competition between energy and proton transfer in ultrafast excited-state dynamics of an oligomeric fluorescent protein red Kaede.", J Phys Chem B, 110(45), 22853-60 (2006)

  101. 101

    Dedecker P, Hotta J, Ando R, Miyawaki A, Engelborghs Y, and Hofkens J: "Fast and reversible photoswitching of the fluorescent protein dronpa as evidenced by fluorescence correlation spectroscopy.", Biophys J, 91(5), L45-7 (2006)

  102. 102

    Kazuno AA, Munakata K, Nagai T, Shimozono S, Tanaka M, Yoneda M, Kato N, Miyawaki A, and Kato T: "Identification of mitochondrial DNA polymorphisms that alter mitochondrial matrix pH and intracellular calcium dynamics.", PLoS Genet, 2(8), e128 (2006)

  103. 103

    Matsu-ura T, Michikawa T, Inoue T, Miyawaki A, Yoshida M, and Mikoshiba K: "Cytosolic inositol 1,4,5-trisphosphate dynamics during intracellular calcium oscillations in living cells.", J Cell Biol, 173(5), 755-65 (2006)

  104. 104

    Habuchi S, Dedecker P, Hotta J, Flors C, Ando R, Mizuno H, Miyawaki A, and Hofkens J: "Photo-induced protonation/deprotonation in the GFP-like fluorescent protein Dronpa: mechanism responsible for the reversible photoswitching.", Photochem Photobiol Sci, 5(6), 567-76 (2006)

  105. 105

    Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, and Miyawaki A: "A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy.", Nat Biotechnol, 24(5), 577-81 (2006)

  106. 106

    Shimozono S, Hosoi H, Mizuno H, Fukano T, Tahara T, and Miyawaki A: "Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer.", Biochemistry, 45(20), 6267-71 (2006)

  107. 107

    Fukano T, Shimozono S, and Miyawaki A: "Fast dual-excitation ratiometry with light-emitting diodes and high-speed liquid crystal shutters.", Biochem Biophys Res Commun, 340(1), 250-5 (2006)

  108. 108

    Kohyama J, Tokunaga A, Fujita Y, Miyoshi H, Nagai T, Miyawaki A, Nakao K, Matsuzaki Y, and Okano H: "Visualization of spatiotemporal activation of Notch signaling: live monitoring and significance in neural development.", Dev Biol, 286(1), 311-25 (2005)

  109. 109

    Miyawaki A: "Innovations in the imaging of brain functions using fluorescent proteins.", Neuron, 48(2), 189-99 (2005)

  110. 110

    Habuchi S, Ando R, Dedecker P, Verheijen W, Mizuno H, Miyawaki A, and Hofkens J: "Reversible single-molecule photoswitching in the GFP-like fluorescent protein Dronpa.", Proc Natl Acad Sci U S A, 102(27), 9511-6 (2005)

  111. 111

    Lin X, Várnai P, Csordás G, Balla A, Nagai T, Miyawaki A, Balla T, and Hajnóczky G: "Control of calcium signal propagation to the mitochondria by inositol 1,4,5-trisphosphate-binding proteins.", J Biol Chem, 280(13), 12820-32 (2005)

  112. 112

    Tsutsui H, Karasawa S, Shimizu H, Nukina N, and Miyawaki A: "Semi-rational engineering of a coral fluorescent protein into an efficient highlighter.", EMBO Rep, 6(3), 233-8 (2005)

  113. 113

    Takao K, Okamoto K, Nakagawa T, Neve RL, Nagai T, Miyawaki A, Hashikawa T, Kobayashi S, and Hayashi Y: "Visualization of synaptic Ca2+ /calmodulin-dependent protein kinase II activity in living neurons.", J Neurosci, 25(12), 3107-12 (2005)

  114. 114

    Wang T, Sakai Y, Nakajima K, Miyawaki A, Ito K, and Hara M: "Nanorheology measurement on single circularly permuted green fluorescent protein molecule.", Colloids Surf B Biointerfaces, 40(3-4), 183-7 (2005)

  115. 115

    Miyawaki A, Nagai T, and Mizuno H: "Engineering fluorescent proteins.", Adv Biochem Eng Biotechnol, 95, 1/15/11 (2005)

  116. 116

    Iwano M, Shiba H, Miwa T, Che FS, Takayama S, Nagai T, Miyawaki A, and Isogai A: "Ca2+ dynamics in a pollen grain and papilla cell during pollination of Arabidopsis.", Plant Physiol, 136(3), 3562-71 (2004)

  117. 117

    Miyawaki A: "Fluorescent proteins in a new light.", Nat Biotechnol, 22(11), 1374-6 (2004)

  118. 118

    Ando R, Mizuno H, and Miyawaki A: "Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting.", Science, 306(5700), 1370-3 (2004)

  119. 119

    Okamoto K, Nagai T, Miyawaki A, and Hayashi Y: "Rapid and persistent modulation of actin dynamics regulates postsynaptic reorganization underlying bidirectional plasticity.", Nat Neurosci, 7(10), 1104-12 (2004)

  120. 120

    Karasawa S, Araki T, Nagai T, Mizuno H, and Miyawaki A: "Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer.", Biochem J, 381(Pt 1), 307-12 (2004)

  121. 121

    Fukano T, Hama H, and Miyawaki A: "Similar diffusibility of membrane proteins across the axon-soma and dendrite-soma boundaries revealed by a novel FRAP technique.", J Struct Biol, 147(1), 12/8/11 (2004)

  122. 122

    Nagai T, Yamada S, Tominaga T, Ichikawa M, and Miyawaki A: "Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins.", Proc Natl Acad Sci U S A, 101(29), 10554-9 (2004)

  123. 123

    Kimura KD, Miyawaki A, Matsumoto K, and Mori I: "The C. elegans thermosensory neuron AFD responds to warming.", Curr Biol, 14(14), 1291-5 (2004)

  124. 124

    Chen J, Kawano H, Nabekawa Y, Mizuno H, Miyawaki A, Tanabe T, Kannari F, and Midorikawa K: "Selective excitation between two-photon and three-photon fluorescence with engineered cost functions.", Opt Express, 12(15), 3408-14 (2004)

  125. 125

    Nagai T, and Miyawaki A: "A high-throughput method for development of FRET-based indicators for proteolysis.", Biochem Biophys Res Commun, 319(1), 1972-7 (2004)

  126. 126

    Hasan MT, Friedrich RW, Euler T, Larkum ME, Giese G, Both M, Duebel J, Waters J, Bujard H, Griesbeck O, Tsien RY, Nagai T, Miyawaki A, and Denk W: "Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control.", PLoS Biol, 2(6), e163 (2004)

  127. 127

    Shimozono S, Fukano T, Kimura KD, Mori I, Kirino Y, and Miyawaki A: "Slow Ca2+ dynamics in pharyngeal muscles in Caenorhabditis elegans during fast pumping.", EMBO Rep, 5(5), 521-6 (2004)

  128. 128

    Fukano T, Shimozono S, and Miyawaki A: "Simultaneous dual-excitation ratiometry using orthogonal linear polarized lights.", Biochem Biophys Res Commun, 317(1), 77-83 (2004)

  129. 129

    Kasai H, Yao A, Oyama T, Hasegawa H, Akazawa H, Toko H, Nagai T, Kinugawa K, Kohmoto O, Maruyama K, Takahashi T, Nagai R, Miyawaki A, and Komuro I: "Direct measurement of Ca2+ concentration in the SR of living cardiac myocytes.", Biochem Biophys Res Commun, 314(4), 1014-20 (2004)

  130. 130

    Hama H, Hara C, Yamaguchi K, and Miyawaki A: "PKC signaling mediates global enhancement of excitatory synaptogenesis in neurons triggered by local contact with astrocytes.", Neuron, 41(3), 405-15 (2004)

  131. 131

    Kawano H, Nabekawa Y, Suda A, Oishi Y, Mizuno H, Miyawaki A, and Midorikawa K: "Attenuation of photobleaching in two-photon excitation fluorescence from green fluorescent protein with shaped excitation pulses.", Biochem Biophys Res Commun, 311(3), 592-6 (2003)

  132. 132

    Mizuno H, Mal TK, Tong KI, Ando R, Furuta T, Ikura M, and Miyawaki A: "Photo-induced peptide cleavage in the green-to-red conversion of a fluorescent protein.", Mol Cell, 12(4), 1051-8 (2003)

  133. 133

    Miyawaki A, Nagai T, and Mizuno H: "Mechanisms of protein fluorophore formation and engineering.", Curr Opin Chem Biol, 7(5), 557-62 (2003)

  134. 134

    Miyawaki A: "Fluorescence imaging of physiological activity in complex systems using GFP-based probes.", Curr Opin Neurobiol, 13(5), 591-6 (2003)

  135. 135

    Karasawa S, Araki T, Yamamoto-Hino M, and Miyawaki A: "A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling.", J Biol Chem, 278(36), 34167-71 (2003)

  136. 136

    Miyawaki A, Sawano A, and Kogure T: "Lighting up cells: labelling proteins with fluorophores.", Nat Cell Biol, Suppl, S1-7 (2003)

  137. 137

    Miyawaki A: "Fluorescent proteins found in cnidarian animals.", Tanpakushitsu Kakusan Koso, 48(11 Suppl), 1568-72 (2003)

  138. 138

    Miyawaki A, Matheson JM, Sayers LG, Muto A, Michikawa T, Furuichi T, and Mikoshiba K.: "Expression of green fluorescent protein and inositol 1,4,5-triphosphate receptor in Xenopus laevis oocytes.", Methods Enzymol, 302, 225-33 (2003)

  139. 139

    Fukano T, and Miyawaki A: "Whole-field fluorescence microscope with digital micromirror device: imaging of biological samples.", Appl Opt, 42(19), 4119-24 (2003)

  140. 140

    Ikeda M, Sugiyama T, Wallace CS, Gompf HS, Yoshioka T, Miyawaki A, and Allen CN: "Circadian dynamics of cytosolic and nuclear Ca2+ in single suprachiasmatic nucleus neurons.", Neuron, 38(2), 253-63 (2003)

  141. 141

    Miyawaki A: "Visualization of the spatial and temporal dynamics of intracellular signaling.", Dev Cell, 4(3), 295-305 (2003)

  142. 142

    Takemoto K, Nagai T, Miyawaki A, and Miura M: "Spatio-temporal activation of caspase revealed by indicator that is insensitive to environmental effects.", J Cell Biol, 160(2), 235-43 (2003)

  143. 143

    Miyawaki A, Mizuno H, Nagai T, and Sawano A: "Development of genetically encoded fluorescent indicators for calcium.", Methods Enzymol, 360, 202-25 (2003)

  144. 144

    Rekas A, Alattia JR, Nagai T, Miyawaki A, and Ikura M: "Crystal structure of venus, a yellow fluorescent protein with improved maturation and reduced environmental sensitivity.", J Biol Chem, 277(52), 50573-8 (2002)

  145. 145

    Ando R, Hama H, Yamamoto-Hino M, Mizuno H, and Miyawaki A: "An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein.", Proc Natl Acad Sci U S A, 99(20), 12651-6 (2002)

  146. 146

    Miyawaki A: "Green fluorescent protein-like proteins in reef Anthozoa animals.", Cell Struct Funct, 27(5), 343-7 (2002)

  147. 147

    Sakamoto K, Yamaguchi S, Ando R, Miyawaki A, Kabasawa Y, Takagi M, Li CL, Perbal B, and Katsube K: "The nephroblastoma overexpressed gene (NOV/ccn3) protein associates with Notch1 extracellular domain and inhibits myoblast differentiation via Notch signaling pathway.", J Biol Chem, 277(33), 29399-405 (2002)

  148. 148

    Sawano A, Takayama S, Matsuda M, and Miyawaki A: "Lateral propagation of EGF signaling after local stimulation is dependent on receptor density.", Dev Cell, 3(2), 245-57 (2002)

  149. 149

    Shimozono S, Fukano T, Nagai T, Kirino Y, Mizuno H, and Miyawaki A: "Confocal imaging of subcellular Ca2+ concentrations using a dual-excitation ratiometric indicator based on green fluorescent protein.", Sci STKE, 2002(125), pl4 (2002)

  150. 150

    Sawano A, Hama H, Saito N, and Miyawaki A: "Multicolor imaging of Ca(2+) and protein kinase C signals using novel epifluorescence microscopy.", Biophys J, 82(2), 1076-85 (2002)

  151. 151

    Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, and Miyawaki A: "A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications.", Nat Biotechnol, 20(1), 87-90 (2002)

  152. 152

    Truong K, Sawano A, Mizuno H, Hama H, Tong KI, Mal TK, Miyawaki A, and Ikura M.: "FRET-based in vivo Ca2+ imaging by a new calmodulin-GFP fusion molecule.", Nat Struct Biol, 8(12), 1069-73 (2001)

  153. 153

    Robert V, Gurlini P, Tosello V, Nagai T, Miyawaki A, Di Lisa F, and Pozzan T.: "Beat-to-beat oscillations of mitochondrial [Ca2+] in cardiac cells.", EMBO J, 20(17), 4998-5007 (2001)

  154. 154

    Kitaguchi T, Nakata K, Nagai T, Aruga J, and Mikoshiba K.: "Xenopus Polycomblike 2 (XPcl2) controls anterior to posterior patterning of the neural tissue.", Dev Genes Evol, 211(6), 309-14 (2001)

  155. 155

    Mochizuki N, Yamashita S, Kurokawa K, Ohba Y, Nagai T, Miyawaki A, and Matsuda M.: "Spatio-temporal images of growth-factor-induced activation of Ras and Rap1.", Nature, 411(6841), 1065-8 (2001)

  156. 156

    Kurokawa K, Mochizuki N, Ohba Y, Mizuno H, Miyawaki A, and Matsuda M.: "A pair of fluorescent resonance energy transfer-based probes for tyrosine phosphorylation of the CrkII adaptor protein in vivo.", J Biol Chem, 276(33), 31305-10 (2001)

  157. 157

    Mizuno H, Sawano A, Eli P, Hama H, and Miyawaki A.: "Red fluorescent protein from Discosoma as a fusion tag and a partner for fluorescence resonance energy transfer.", Biochemistry, 40(8), 10月-02 (2001)

  158. 158

    Nagai T, Sawano A, Park ES, and Miyawaki A.: "Circularly permuted green fluorescent proteins engineered to sense Ca2+.", Proc Natl Acad Sci U S A, 98(6), 3197-202 (2001)

  159. 159

    Kitaguchi T, Nagai T, Nakata K, Aruga J, and Mikoshiba K.: "Zic3 is involved in the left-right specification of the Xenopus embryo.", Development, 127(22), 4787-95 (2000)

  160. 160

    Sawano A, and Miyawaki A.: "Directed evolution of green fluorescent protein by a new versatile PCR strategy for site-directed and semi-random mutagenesis.", Nucleic Acids Res, 28(16), E78 (2000)

  161. 161

    Nagai T, Aruga J, Minowa O, Sugimoto T, Ohno Y, Noda T, and Mikoshiba K.: "Zic2 regulates the kinetics of neurulation.", Proc Natl Acad Sci U S A, 97(4), 1618-23 (2000)

  162. 162

    Matsushita F, Miyawaki A, and Mikoshiba K.: "Vomeroglandin/CRP-Ductin is strongly expressed in the glands associated with the mouse vomeronasal organ: identification and characterization of mouse vomeroglandin.", Biochem Biophys Res Commun, 268(2), 275-81 (2000)

  163. 163

    Tamura H, Miyawaki A, Yoneshima H, Mikoshiba K, and Matsui M.: "Molecular cloning, expression and characterization of a phenol sulfotransferase cDNA from mouse intestine.", Biol Pharm Bull, 22(3), 234-9 (1999)